Setup

FastQC Summary

Summary statistics for all libraries
Filename Total Flagged As Poor Quality Sequence Length %GC
313A_C85FPANXX_CGTACG_L004_R1.fastq 30,180,462 0 35-100 47
313A_C85FPANXX_CGTACG_L004_R2.fastq 30,180,462 0 35-100 47
313B_C85FPANXX_GAGTGG_L004_R1.fastq 30,289,816 0 35-100 47
313B_C85FPANXX_GAGTGG_L004_R2.fastq 30,289,816 0 35-100 47
313C_C85FPANXX_ACTGAT_L004_R1.fastq 29,578,786 0 35-100 47
313C_C85FPANXX_ACTGAT_L004_R2.fastq 29,578,786 0 35-100 47
352A_C85FPANXX_ATTCCT_L004_R1.fastq 29,015,015 0 35-100 47
352A_C85FPANXX_ATTCCT_L004_R2.fastq 29,015,015 0 35-100 47
352B_C85FPANXX_ATCACG_L004_R1.fastq 28,249,736 0 35-100 46
352B_C85FPANXX_ATCACG_L004_R2.fastq 28,249,736 0 35-100 46
352C_C85FPANXX_CGATGT_L004_R1.fastq 28,528,176 0 35-100 46
352C_C85FPANXX_CGATGT_L004_R2.fastq 28,528,176 0 35-100 47
58722A_R1.fastq 22,776,350 0 35-100 46
58722A_R2.fastq 22,776,350 0 35-100 46
58722B_R1.fastq 25,487,151 0 35-100 46
58722B_R2.fastq 25,487,151 0 35-100 46
58722C_R1.fastq 26,773,933 0 35-100 46
58722C_R2.fastq 26,773,933 0 35-100 46

Read Totals

Library Sizes ranged between 22,776,350 and 30,289,816 reads.

Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.

FastQC Summary

Summary of FastQC flags for each parameter

Per Base Sequence Quality

Heatmap showing mean base qualities for each library

Per Sequence Quality Scores

Heatmap showing mean sequence qualities for each library

Per Base Sequence Content

Heatmap of summed base distributions along each read

Per Sequence GC Content

GC Content Heatmap normalised to theoretical GC content in the Hsapiens Transcriptome

GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Transcriptome

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented Sequences

Summary of Overrepresented Sequences in all files. A maximum of 30 seqences are shown.
Filename Sequence Count Percentage Possible_Source
313A_C85FPANXX_CGTACG_L004_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 49,322 0.1634 No Hit
313B_C85FPANXX_GAGTGG_L004_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 59,832 0.1975 No Hit
313C_C85FPANXX_ACTGAT_L004_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 43,797 0.1481 No Hit
352A_C85FPANXX_ATTCCT_L004_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 68,067 0.2346 No Hit
352B_C85FPANXX_ATCACG_L004_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 53,390 0.189 No Hit
352C_C85FPANXX_CGATGT_L004_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 46,101 0.1616 No Hit
58722A_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 36,962 0.1623 No Hit
58722B_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 42,496 0.1667 No Hit
58722C_R2 AAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCT 42,557 0.1589 No Hit
313B_C85FPANXX_GAGTGG_L004_R2 AAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTC 33,655 0.1111 No Hit
352A_C85FPANXX_ATTCCT_L004_R2 AAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTC 38,048 0.1311 No Hit
352B_C85FPANXX_ATCACG_L004_R2 AAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTC 30,033 0.1063 No Hit
58722A_R2 AATGGACCCTACTGCAGAAATATCAAAAAGAAGTGTCTACGTTTTGTGTG 25,048 0.11 No Hit
58722B_R2 AATGGACCCTACTGCAGAAATATCAAAAAGAAGTGTCTACGTTTTGTGTG 27,086 0.1063 No Hit
58722C_R2 AATGGACCCTACTGCAGAAATATCAAAAAGAAGTGTCTACGTTTTGTGTG 27,955 0.1044 No Hit
313A_C85FPANXX_CGTACG_L004_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 69,007 0.2286 No Hit
313B_C85FPANXX_GAGTGG_L004_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 80,672 0.2663 No Hit
313C_C85FPANXX_ACTGAT_L004_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 60,751 0.2054 No Hit
352A_C85FPANXX_ATTCCT_L004_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 88,964 0.3066 No Hit
352B_C85FPANXX_ATCACG_L004_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 82,565 0.2923 No Hit
352C_C85FPANXX_CGATGT_L004_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 70,536 0.2473 No Hit
58722A_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 44,680 0.1962 No Hit
58722B_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 50,854 0.1995 No Hit
58722C_R2 AATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCCCTCT 53,969 0.2016 No Hit
313A_C85FPANXX_CGTACG_L004_R2 ACAAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCC 43,166 0.143 No Hit
313B_C85FPANXX_GAGTGG_L004_R2 ACAAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCC 54,635 0.1804 No Hit
313C_C85FPANXX_ACTGAT_L004_R2 ACAAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCC 43,332 0.1465 No Hit
352A_C85FPANXX_ATTCCT_L004_R2 ACAAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCC 64,079 0.2208 No Hit
352B_C85FPANXX_ATCACG_L004_R2 ACAAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCC 60,059 0.2126 No Hit
352C_C85FPANXX_CGATGT_L004_R2 ACAAAATGTATCCTGCTCACCTTCTGGTCCTGTTGGCAGTTTGTGTCTCC 49,537 0.1736 No Hit

Adapter Content

Universal Adapter Content

Kmer Content

Summary of Overrepresented Kmers in all files. A maximum of 30 Kmers are shown.
Filename Sequence Count PValue Obs/Exp_Max Max_Obs/Exp_Position
352A_C85FPANXX_ATTCCT_L004_R2 ACTGACA 126,780 0 27.16 8
313B_C85FPANXX_GAGTGG_L004_R2 ACTGACA 123,300 0 27.15 8
352B_C85FPANXX_ATCACG_L004_R2 ACTGACA 122,690 0 30.74 8
352A_C85FPANXX_ATTCCT_L004_R2 CACTGAC 121,480 0 28.34 7
352B_C85FPANXX_ATCACG_L004_R2 CACTGAC 118,175 0 31.9 7
313B_C85FPANXX_GAGTGG_L004_R2 CACTGAC 115,320 0 29.01 7
352B_C85FPANXX_ATCACG_L004_R2 CCACTGA 113,140 0 33.62 6
313A_C85FPANXX_CGTACG_L004_R1 CCAGAGA 123,870 0 25.33 8
313B_C85FPANXX_GAGTGG_L004_R1 CCAGAGA 115,045 0 25.98 8
352A_C85FPANXX_ATTCCT_L004_R2 CTGACAA 136,420 0 25.33 9
313B_C85FPANXX_GAGTGG_L004_R2 CTGACAA 133,115 0 25.22 9
352B_C85FPANXX_ATCACG_L004_R2 CTGACAA 131,465 0 28.74 9
58722C_R2 CTGACAA 120,040 0 25.93 9
58722B_R2 CTGACAA 115,845 0 25.4 9
352A_C85FPANXX_ATTCCT_L004_R2 GACAAAA 158,635 0 16.04 5
313B_C85FPANXX_GAGTGG_L004_R2 GACAAAA 154,105 0 16.03 5
58722C_R2 GACAAAA 133,735 0 16.53 5
58722B_R2 GACAAAA 130,530 0 16.03 5
313A_C85FPANXX_CGTACG_L004_R2 GACAAAA 128,730 0 15.62 5
313A_C85FPANXX_CGTACG_L004_R1 TCCAGAG 125,315 0 25.14 7
313B_C85FPANXX_GAGTGG_L004_R1 TCCAGAG 120,140 0 24.95 7
352A_C85FPANXX_ATTCCT_L004_R2 TGACAAA 147,470 0 17.2 4
313B_C85FPANXX_GAGTGG_L004_R2 TGACAAA 145,555 0 17.01 4
58722C_R2 TGACAAA 126,250 0 17.43 4
313A_C85FPANXX_CGTACG_L004_R2 TGACAAA 124,125 0 16.22 4
352C_C85FPANXX_CGATGT_L004_R2 TGACAAA 122,940 0 17.18 4
58722B_R2 TGACAAA 122,135 0 17.08 4
58722C_R2 TGCGCGT 131,290 0 25.1 94
352B_C85FPANXX_ATCACG_L004_R2 TGCGCGT 117,810 0 31.78 94
58722B_R2 TGCGCGT 114,565 0 25.28 94